Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone NKI/M6] (IgG1) to Human Melanoma-Associated Antigen
IHC, IHC - Frozen, Immunofluorescence
Human Melanoma-Associated Antigen
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [NKI/M6]
IHC - Frozen (1:5 - 1:10)
Specificity and Use
Melanoma Associated Antigen.
Recognizes the Melanoma Associated Antigen, a high molecular weight proteoglycan with a molecular weight of >450kD (chondroitin sulfate) and 250kD (core protein). Reacts strongly with melanoma cells derived from cell lines and short term cultures. Reacts with most nevi and perineurium, and shows weak reactivity with hair follicles.
Suitable for use in Immunohistochemistry. Can also be used to detect melanoma lesions in vivo. Immunohistochemistry (Frozen): 1:5-1:10 diluted in PBS. Stain by regular immunoperoxidase and immunofluorescence tests. Reacts preferentially with melanoma cells in frozen tissue sections.
PBS, 0.1% BSA
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.