Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [NKI-C3]
IHC - Paraffin (10 µg/ml)
IHC - Frozen
Specificity and Use
Used for diagnostic by staining of lysosomes/melanosomes. Directed against a predominantly intracellular protein of human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three V-linked glycans were added cotranslationally. Extensive further heterogeneity was generated in the Colgi compartmen and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with cathepsin D, which is a marker for lysosomal functions.This antibody was originally described as a marker for melanoma. Recently, it resurfaced as a marker to separate cellular neurothekeoma from other dermal tumors in the differential diagnosis.
PBS containing 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.