Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone NKI/beteb] (IgG2b) to Human Melanoma-Associated Antigen
IHC - Paraffin, IHC - Frozen, Immunoprecipitation
Human Melanoma-Associated Antigen
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [NKI/beteb]
IHC - Paraffin
IHC - Frozen
Specificity and Use
This antibody recognizes a (pre)melanosomal 100 + 7 kd antigen (glycoprotein). The antibody reacts with melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non-Hodgkin's lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
For immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.