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| Catalog Number | Size | Price |
|---|---|---|
| LS-C25535-100 | 100 µl | $717 |
Polyclonal Rabbit anti‑Human MEK1 + MEK2 Antibody (phospho‑Ser217/Ser221, WB) LS‑C25535
Polyclonal Rabbit anti‑Human MEK1 + MEK2 Antibody (phospho‑Ser217/Ser221, WB) LS‑C25535
Antibody:
MEK1 + MEK2 Rabbit anti-Human Polyclonal (pSer217/Ser221) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MEK1 + MEK2 Rabbit anti-Human Polyclonal (pSer217/Ser221) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
MEK1 + MEK2 antibody LS-C25535 is an unconjugated rabbit polyclonal antibody to MEK1 + MEK2 (pSer217/Ser221) from human. It is reactive with human, mouse and rat. Validated for IP and WB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.
Epitope
pSer217/Ser221
Specificity
Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:50)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Dog, Rabbit, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Dog, Pig, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Porcine
Range:
0.31-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
