LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-MED27 / CRSP8 Antibody (clone 8B8) LS-C133589


Wt. Vol. Conc. Price
50 µg - - Unavailable

Related Products

34 MED27 / CRSP8 Antibodies

Most Popular MED27 / CRSP8 Antibodies

Anti-MED27 / CRSP8 Antibody (C-Terminus) LS-C30749
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Western blot
Western blot Image
Anti-MED27 / CRSP8 Antibody (aa180-229) LS-C109825
Rabbit Polyclonal to Human MED27 / CRSP8
Human, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus
Western blot
Western blot Image
Anti-MED27 / CRSP8 Antibody (clone 8B8) LS-C133589
Mouse Monoclonal [clone 8B8] (IgG1,k) to Human MED27 / CRSP8
Western blot, ELISA
Western blot Image
Anti-MED27 / CRSP8 Antibody (aa90-170) LS-C382912
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Human, Mouse
Western blot, ELISA
Anti-MED27 / CRSP8 Antibody LS-C399336
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8B8] (IgG1,k) to Human MED27 / CRSP8
Western blot, ELISA


Human MED27 / CRSP8
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8B8]
Purified from ascites by Protein A


  • Western blot

  • IHC - Paraffin

Specificity and Use

MED27 / CRSP8 antibody was raised against cRSP8 (AAH02878, 1 a.a. ~ 71 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MED27
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.


PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About MED27 / CRSP8

Q6P2C8 NM_004269 NP_004260.2

MED27 Antibody, CRAP34 Antibody, CRSP complex subunit 8 Antibody, Mediator complex subunit 27 Antibody, TRAP37 Antibody, p37 TRAP/SMCC/PC2 subunit Antibody, CRSP34 Antibody, CRSP8 Antibody

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g.

Western blot

Western blot
Western blot of CRSP8 expression in transfected 293T cell line by CRSP8 monoclonal antibody (M01), clone 8B8.

Western blot

Western blot
CRSP8 monoclonal antibody (M01), clone 8B8 Western blot of CRSP8 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MED27 is 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/26/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number