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Anti-MED27 / CRSP8 Antibody (clone 8B8) LS-C133589

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Related Products

34 MED27 / CRSP8 Antibodies

Most Popular MED27 / CRSP8 Antibodies

Anti-MED27 / CRSP8 Antibody (C-Terminus) LS-C30749
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Western blot
Unconjugated
Western blot Image
Anti-MED27 / CRSP8 Antibody (aa180-229) LS-C109825
Rabbit Polyclonal to Human MED27 / CRSP8
Human, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus
Western blot
Unconjugated
Western blot Image
Anti-MED27 / CRSP8 Antibody (clone 8B8) LS-C133589
Mouse Monoclonal [clone 8B8] (IgG1,k) to Human MED27 / CRSP8
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-MED27 / CRSP8 Antibody (aa90-170) LS-C382912
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Human, Mouse
Western blot, ELISA
Unconjugated
Anti-MED27 / CRSP8 Antibody LS-C399336
Rabbit Polyclonal (IgG) to Human MED27 / CRSP8
Human
Western blot, ELISA
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Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8B8] (IgG1,k) to Human MED27 / CRSP8
Human
Western blot, ELISA
Unconjugated

Details

Human MED27 / CRSP8
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8B8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

MED27 / CRSP8 antibody was raised against cRSP8 (AAH02878, 1 a.a. ~ 71 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MED27
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About MED27 / CRSP8

Q6P2C8 NM_004269 NP_004260.2

MED27 Antibody, CRAP34 Antibody, CRSP complex subunit 8 Antibody, Mediator complex subunit 27 Antibody, TRAP37 Antibody, p37 TRAP/SMCC/PC2 subunit Antibody, CRSP34 Antibody, CRSP8 Antibody

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g.

Western blot

Western blot
Western blot of CRSP8 expression in transfected 293T cell line by CRSP8 monoclonal antibody (M01), clone 8B8.

Western blot

Western blot
CRSP8 monoclonal antibody (M01), clone 8B8 Western blot of CRSP8 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MED27 is 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/26/2017

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