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MED14 Antibody (aa575‑624) LS‑C116775
MED14 antibody LS-C116775 is an unconjugated rabbit polyclonal antibody to MED14 from human, mouse, monkey and other species. Validated for WB.
Catalog
Size
Price
LS-C116775-50
50 µg
Unavailable

Popular MED14 Products

Antibody
Species: Human
Applications: Western blot
Immunofluorescence of COS7 cells, using MED14 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
MED14 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The MED14 antibody detected the MED14 protein (arrow).
Species: Human, Mouse
Applications: Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, ELISA
Western blot of MED14 antibody
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot

Product Description

MED14 antibody LS-C116775 is an unconjugated rabbit polyclonal antibody to MED14 from human, mouse, monkey and other species. Validated for WB.
About MED14
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. O60244 NM_004229 NP_004220.2

MED14 Antibody, CSRP Antibody, CRSP2 Antibody, CXorf4 Antibody, CRSP150 Antibody, DRIP150 Antibody, EXLM1 Antibody, Human homolog of yeast RGR1 Antibody, HRGR1 Antibody, Mediator complex subunit 14 Antibody, TRAP170 Antibody, RGR1 homolog Antibody, ARC150 Antibody, CRSP complex subunit 2 Antibody, RGR1 Antibody


Specifications

Target
Human MED14
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MED14 antibody was raised against synthetic peptide located between aa575-624 of human MED14 (O60244, NP_004220). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Rabbit (85%); Mouse (78%).
Blocking Peptide
LS-E21277 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116775. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa575-624
Specificity
Human MED14
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MED14 antibody LS-C116775 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MED14 antibody LS-C116775 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MED14 antibody LS-C116775 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MED14 antibody LS-C116775 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MED14 antibody LS-C116775 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MED14 antibody LS-C116775 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MED14 antibody LS-C116775 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MED14 antibody LS-C116775 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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