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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MED1 / TRAP220 Antibody (clone 2A2) LS‑C197960
TRAP220 antibody LS-C197960 is an unconjugated mouse monoclonal antibody to human TRAP220 (MED1). Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C197960-100
100 µg
Unavailable

Popular MED1 / TRAP220 Products

Immunofluorescence of HeLa cells, using PPAR-BP (Ab-1457) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of extracts from Jurkat cells, using MED1 Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-CRSP1/TRAP220 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Applications: Western blot, Immunoprecipitation, Proximity Ligation Assay
Immunohistochemical analysis of TRAP220 (pT1457) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

TRAP220 antibody LS-C197960 is an unconjugated mouse monoclonal antibody to human TRAP220 (MED1). Validated for ELISA, IF and WB.
About MED1 / TRAP220
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. Q15648 NM_004774 NP_004765.2

MED1 Antibody, ARC205 Antibody, CRSP1 Antibody, DRIP205 Antibody, DRIP230 Antibody, Mediator complex subunit 1 Antibody, PBP Antibody, PPARG binding protein Antibody, RB18A Antibody, TRIP-2 Antibody, TRIP2 Antibody, PPARBP Antibody, CRSP200 Antibody, p53 regulatory protein RB18A Antibody, PPAR binding protein Antibody, PPAR-binding protein Antibody, PPARGBP Antibody, TR-interacting protein 2 Antibody, TRAP220 Antibody


Specifications

Target
Human MED1 / TRAP220
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2A2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
MED1 / TRAP220 antibody was raised against pPARBP (NP_004765, aa1391-1490 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human MED1 / TRAP220
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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