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Anti-MED1 / TRAP220 Antibody (clone 2A2) LS-C197960

Catalog Size Price
LS-C197960-100 100 µg Unavailable

Related Products

25 MED1 / TRAP220 Antibodies

Most Popular MED1 / TRAP220 Antibodies

Anti-MED1 / TRAP220 Antibody (aa1423-1472) LS-C117699
Rabbit Polyclonal (IgG) to Human MED1 / TRAP220
Human, Mouse
Immunofluorescence, ELISA
Unconjugated
Immunofluorescence of HeLa cells, using PPAR-BP (Ab-1457) Antibody. The picture on the right is treated with the synthesized peptide.
Anti-MED1 / TRAP220 Antibody (aa641-690) LS-C119067
Rabbit Polyclonal (IgG) to Human MED1 / TRAP220
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Western blot of extracts from Jurkat cells, using MED1 Antibody. The lane on the right is treated with the synthesized peptide.
Anti-MED1 / TRAP220 Antibody (aa1531-1581) LS-C290523
Goat Polyclonal (IgG) to Human MED1 / TRAP220
Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-CRSP1/TRAP220 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Anti-MED1 / TRAP220 Antibody (phospho-Thr1457) LS-C358800
Rabbit Polyclonal to Human MED1 / TRAP220
Human, Monkey, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemical analysis of TRAP220 (pT1457) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MED1 / TRAP220 Antibody (Internal) IHC-plus™ LS-B14558
Rabbit Polyclonal to Human MED1 / TRAP220
Human, Monkey, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

100% Guaranteed
Mouse Monoclonal [clone 2A2] (IgG1,k) to Human MED1 / TRAP220
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human MED1 / TRAP220
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2A2]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

MED1 / TRAP220 antibody was raised against pPARBP (NP_004765, aa1391-1490 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MED1 / TRAP220
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About MED1 / TRAP220

Q15648 NM_004774 NP_004765.2

MED1 Antibody, ARC205 Antibody, CRSP1 Antibody, DRIP205 Antibody, DRIP230 Antibody, Mediator complex subunit 1 Antibody, PBP Antibody, PPARG binding protein Antibody, RB18A Antibody, TRIP-2 Antibody, TRIP2 Antibody, PPARBP Antibody, CRSP200 Antibody, p53 regulatory protein RB18A Antibody, PPAR binding protein Antibody, PPAR-binding protein Antibody, PPARGBP Antibody, TR-interacting protein 2 Antibody, TRAP220 Antibody

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g.

Immunofluorescence

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/22/2018

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