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Anti-ME3 Antibody (aa38-87) LS-C81250

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Wt. Vol. Conc. Price
50 µg 50 µl - Unavailable

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Anti-ME3 Antibody (aa38-87) LS-C81250
Rabbit Polyclonal (IgG) to Human ME3
Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Rabbit, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ME3
Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Rabbit, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ME3
Rabbit
Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Sheep (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

ME3 antibody was raised against synthetic peptide located between aa38-87 of human ME3 (Q16798, NP_001014811). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Rabbit, Opossum, Guinea pig, Xenopus, Zebrafish (100%); Salmon, Stickleback (92%); Sheep (91%); Monkey, Pig (84%).
LS-E17549 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81250. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa38-87
Human ME3
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ME3

Q16798 NM_006680 NP_006671.2

ME3 Antibody, Malate dehydrogenase Antibody, NADP-ME Antibody, Pyruvic-malic carboxylase Antibody, Malic enzyme 3 Antibody

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/30/2017

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