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ME1 / Malate Dehydrogenase Antibody (aa36‑85) LS‑C101411
Malate Dehydrogenase antibody LS-C101411 is an unconjugated rabbit polyclonal antibody to Malate Dehydrogenase (ME1) from human, dog and pig. Validated for WB.
Catalog
Size
Price
LS-C101411-50
50 µg
Unavailable

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Product Description

Malate Dehydrogenase antibody LS-C101411 is an unconjugated rabbit polyclonal antibody to Malate Dehydrogenase (ME1) from human, dog and pig. Validated for WB.
About ME1 / Malate Dehydrogenase
ME1 / Malate Dehydrogenase encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. P48163 NM_002395 NP_002386.1

ME1 Antibody, HUMNDME Antibody, Malate dehydrogenase Antibody, Malic enzyme 1 Antibody, Malate oxidoreductase Antibody, Malic enzyme 1, soluble Antibody, MES Antibody, NADP-ME Antibody, Pyruvic-malic carboxylase Antibody, NADP-dependent malic enzyme Antibody, Malic enzyme, cytoplasmic Antibody


Specifications

Target
Human ME1 / Malate Dehydrogenase
Host
Rabbit
Reactivity
Human, Dog, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Horse, Sheep, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa36-85 of human ME1 (P48163, NP_002386). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%); Monkey, Galago, Marmoset, Sheep, Bovine, Horse (92%); Bat, Opossum, Chicken, Platypus, Lizard (90%); Elephant, Dog, Pig, Stickleback (84%).
Blocking Peptide
LS-E11544 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101411. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human ME1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ME1 antibody LS-C101411 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ME1 antibody LS-C101411 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ME1 antibody LS-C101411 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ME1 antibody LS-C101411 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ME1 antibody LS-C101411 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ME1 antibody LS-C101411 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ME1 antibody LS-C101411 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ME1 antibody LS-C101411 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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