(tested or 100% immunogen sequence identity)
- Western blot (1:10000 - 1:25000)
- Flow Cytometry
MDM4 / MDMX antibody was raised against a synthetic peptide which maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in Swiss-Prot entry O15151 (GeneID 4194).
Specific for human HdmX/MDM4 protein. Other species not tested. Reactivity with mouse MdmX is unlikely owing to divergence of sequence between MdmX and HdmX/MDM4 at the site to which the epitope maps.
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Immunoprecipitation: 2-5 ug/mg lysate. Western Blot: 1:10000-1:25000.
Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
For research use only.
This antibody carries the LSBio 100% Guarantee.