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Catalog Number Size Price
LS-C25501-50 50 µg $778 
Antibody

Monoclonal Mouse anti‑Human MDM2 Antibody (aa26‑169, IHC, IF, WB) LS‑C25501

Monoclonal Mouse anti‑Human MDM2 Antibody (aa26‑169, IHC, IF, WB) LS‑C25501

Antibody:
MDM2 Mouse anti-Human Monoclonal (aa26-169) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$778
LS-C25501-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MDM2 Mouse anti-Human Monoclonal (aa26-169) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MDM2 antibody LS-C25501 is an unconjugated mouse monoclonal antibody to human MDM2 (aa26-169). Validated for IF, IHC, IP and WB.
Target
Human MDM2
Synonyms
MDM2 | ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
A synthetic peptide derived from human MDM2 protein (1).
Epitope
aa26-169
Specificity
Recognizes human MDM2 protein. Does not react with mouse MDM2. Epitope recognized by this antibody is located within amino acids 26-169 of the human protein.
Applications
  • IHC
  • IHC - Paraffin (1 - 5 µg/ml)
  • IHC - Frozen (1 - 5 µg/ml)
  • Immunofluorescence (1 - 5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Western Blot: 0.5-2 ug/ml (chemiluminescence detection). Immunohistochemistry (frozen): 1-5 ug/ml. Immunohistochemistry (formalin-fixed, paraffin-embedded): 1-5 ug/ml. Staining requires heat induced epitope retrieval (HIER) pretreatment. Immunoprecipitation: 1 ug/sample (autoradiographic detection). Positive control: OsA-CL, MCF-7 and HeLa cell lysates.
Presentation
PBS, pH 7.4, 0.05% sodium azide, 1 mg/ml BSA, 30% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MDM2
Q00987 NM_002392 NP_002383.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024