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Anti-MDM2 Antibody (aa177-195) LS-C19046

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MDM2 Antibodies

Anti-MDM2 Antibody (aa154-167, clone SMP14) IHC-plus™ LS-B163
Mouse Monoclonal [clone SMP14] (IgG1) to Human MDM2
Human, Monkey, Rat, Dog
IHC - Paraffin, IHC - Frozen, IHC - Resin, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-MDM2 Antibody (clone 1A7) IHC-plus™ LS-B5461
Mouse Monoclonal [clone 1A7] (IgG1,k) to Human MDM2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MDM2 Antibody (Internal) LS-C209837
Rabbit Polyclonal (IgG) to Zebrafish MDM2
Zebrafish
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse MDM2
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse MDM2
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA (1:3000 - 1:12000)

Specificity and Use

MDM2 antibody was raised against synthetic peptide
aa177-195
aa 177-195 of mouse MDM2.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kD in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.

Packaging

0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About MDM2

Q00987 NM_002392 NP_002383.2

MDM2 Antibody, ACTFS Antibody, Double minute 2 protein Antibody, Hdm2 Antibody, MDM2 variant FB30 Antibody, HDMX Antibody, Oncoprotein Mdm2 Antibody, p53-binding protein Mdm2 Antibody, MDM2 variant FB28 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein.

Western blot

Western blot
Anti-MDM2 Antibody - Western Blot. Western blot using affinity purified Anti-MDM2 (Rabbit) is shown to detect a band (arrow) corresponding to mouse MDM2 protein present in mouse MEF cells (lane 2) but not human kidney HEK293 cells (lane 1). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/10/2016

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