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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MDH1 Antibody (aa286‑314) LS‑C164105
MDH1 antibody LS-C164105 is an unconjugated rabbit polyclonal antibody to human MDH1. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C164105-400
400 µl
Unavailable

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Product Description

MDH1 antibody LS-C164105 is an unconjugated rabbit polyclonal antibody to human MDH1. Validated for Flow, IHC and WB.
About MDH1
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. P40925 NM_005917 NP_005908.1

MDH1 Antibody, Cytosolic malate dehydrogenase Antibody, Diiodophenylpyruvate reductase Antibody, MOR2 Antibody, MDH-s Antibody, MDHA Antibody, MGC:1375 Antibody, Soluble malate dehydrogenase Antibody


Specifications

Target
Human MDH1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Chicken, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7192 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164105. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa286-314
Specificity
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).
MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).

Flow Cytometry

MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).
MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).

Flow Cytometry

MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).
MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).

Flow Cytometry

MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).
MDH1 Antibody western blot of HL-60,THP-1 cell line lysates (35 ug/lane). The MDH1 antibody detected the MDH1 protein (arrow).

Flow Cytometry

MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MDH1 Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/19/2018

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