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Antibodies > MCM6 > LS-C312918
 
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Catalog Number Size Price
LS-C312918-10 10 µg $318 
LS-C312918-100 100 µg (0.5 mg/ml) $470 
MCM6 Antibody - MCM6 antibody. IHC(F): Human Placenta Tissue.
MCM6 Antibody - IHC analysis of MCM6 using anti-MCM6 antibody. MCM6 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MCM6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MCM6 Antibody - MCM6 antibody. IHC(P): Human Intestinal Cancer Tissue.
MCM6 Antibody - MCM6 antibody. IHC(P): Rat Brain Tissue.
MCM6 Antibody - MCM6 antibody. ICC: HELA Cell.
MCM6 Antibody - Western blot analysis of MCM6 using anti-MCM6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates, Lane 2: mouse spleen tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM6 at approximately 93KD. The expected band size for MCM6 is at 93KD.
MCM6 Antibody - WB of MCM6 antibody. All lanes: Anti-MCM6 at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: COLO320 Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 93KD. Observed bind size: 93KD.
MCM6 Antibody - MCM6 antibody. IHC(F): Human Placenta Tissue.
MCM6 Antibody - IHC analysis of MCM6 using anti-MCM6 antibody. MCM6 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MCM6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MCM6 Antibody - MCM6 antibody. IHC(P): Human Intestinal Cancer Tissue.
MCM6 Antibody - MCM6 antibody. IHC(P): Rat Brain Tissue.
MCM6 Antibody - MCM6 antibody. ICC: HELA Cell.
MCM6 Antibody - Western blot analysis of MCM6 using anti-MCM6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates, Lane 2: mouse spleen tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM6 at approximately 93KD. The expected band size for MCM6 is at 93KD.
MCM6 Antibody - WB of MCM6 antibody. All lanes: Anti-MCM6 at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: COLO320 Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 93KD. Observed bind size: 93KD.
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Polyclonal Rabbit anti‑Human MCM6 Antibody (aa589‑605, IHC, WB) LS‑C312918

Polyclonal Rabbit anti‑Human MCM6 Antibody (aa589‑605, IHC, WB) LS‑C312918

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Note: This antibody replaces LS-C388813
Antibody:
MCM6 Rabbit anti-Human Polyclonal (aa589-605) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312918-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MCM6 Rabbit anti-Human Polyclonal (aa589-605) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
MCM6 antibody LS-C312918 is an unconjugated rabbit polyclonal antibody to MCM6 (aa589-605) from human. It is reactive with human and monkey. Validated for ICC, IHC and WB.
Target
Human MCM6
Synonyms
MCM6 | Mis5 | MCG40308 | p105MCM
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MCM6(589-605 aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid.
Epitope
aa589-605
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM6
Q14566 NM_005915 NP_005906.2

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MCM6 Antibody (C‑Terminus, Biotin) LS‑C434551
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Applications: IHC, IHC - Paraffin, Western blot
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Species: Human
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See all MCM6 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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