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Anti-MCM5 Antibody IHC-plus™ LS-B2509

Note: This antibody replaces LS-C40926

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MCM5 Antibodies

Anti-MCM5 Antibody IHC-plus™ LS-B2509
Rabbit Polyclonal (IgG) to Mouse MCM5
Mouse, Human, Monkey, Bat, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MCM5 Antibody (clone CRCT5.1) LS-C122108
Mouse Monoclonal [clone CRCT5.1] (IgG2b) to Human MCM5
Human
IHC - Paraffin, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse MCM5
Mouse, Human, Monkey, Bat, Horse
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse MCM5
Rabbit
Mouse, Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot

Specificity and Use

MCM5 antibody was raised against synthetic peptide from mouse MCM5. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bat, Horse (100%); Elephant, Panda, Turkey, Chicken, Lizard, Pufferfish, Sea anemone (93%); Mouse, Rat, Hamster, Bovine, Rabbit, Xenopus, Salmon, Stickleback, Zebrafish (87%); Seq squirt, Water flea, Beetle (80%).
Peptide-KLH (KEVADEVTRPRPSGE).
Immunohistochemistry: LS-B2509 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2509 was determined to be 1:50.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
For research use only.

About MCM5

P33992 NM_006739 NP_006730.2

MCM5 Antibody, CDC46 Antibody, CDC46 homolog Antibody, p1-CDC46 Antibody

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.

Immunohistochemistry

Immunohistochemistry
Anti-MCM5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2509 dilution 1:50.

Western blot

Western blot
Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Requested From: United States
Date Requested: 12/8/2016

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