Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Monoclonal [clone RBT-MCM5] (IgG) to Human MCM5
IHC - Paraffin, IHC - Frozen
Mouse Monoclonal [clone CRCT5.1] (IgG2b) to Human MCM5
IHC - Paraffin, Western blot
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [CRCT5.1]
IHC - Paraffin (1:25 - 1:100)
Western blot (1:25 - 1:100)
Specificity and Use
MCM5 antibody was raised against bacterially expressed human Mcm5.
Recognizes human MCM5.
Suitable for use in Western Blot and Immunohistochemistry. Immunohistochemistry (Paraffin): 1:25-1:100 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 0.01 M citrate buffer, pH 6 is recommended for this purpose.
PBS, 0.9% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.