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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MCM4 Antibody (Pro50) LS‑C177430
MCM4 antibody LS-C177430 is an unconjugated rabbit polyclonal antibody to MCM4 from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C177430-100
100 µl
Unavailable

Popular MCM4 Products

Detection of Human MCM4 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM4 used at a dilution of 1:1000 (1 Detection: DAB.
Species: Human, Guinea pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Proximity Ligation Assay
Detection of Human and Mouse MCM4 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 used at a dilution of 1:1000 (1 Detection: DAB.
Species: Human, Mouse, Bovine, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Proximity Ligation Assay
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemical analysis of MCM4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Monkey, Mouse
Applications: IHC, Western blot, ELISA

Product Description

MCM4 antibody LS-C177430 is an unconjugated rabbit polyclonal antibody to MCM4 from human and mouse. Validated for IHC and WB.
About MCM4
MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. P33991 X74794 CAA52801.1

MCM4 Antibody, CDC21 homolog Antibody, CDC54 Antibody, HCdc21 Antibody, NKGCD Antibody, p1-CDC21 Antibody, NKCD Antibody, CDC21 Antibody


Specifications

Target
Human MCM4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCM4 antibody was raised against synthetic peptide around Pro50 of human MCM4
Epitope
Pro50
Specificity
Human MCM4
Presentation
PBS, pH 7.2, 15 mM sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.
IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.

Western blot

Western blot of MCM4 (P50) pAb in extracts from HeLa cells.
Western blot of MCM4 (P50) pAb in extracts from HeLa cells.

Immunohistochemistry

IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.
IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.

Western blot

Western blot of MCM4 (P50) pAb in extracts from HeLa cells.
Western blot of MCM4 (P50) pAb in extracts from HeLa cells.

Immunohistochemistry

IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.
IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.

Western blot

Western blot of MCM4 (P50) pAb in extracts from HeLa cells.
Western blot of MCM4 (P50) pAb in extracts from HeLa cells.

Immunohistochemistry

IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.
IHC of MCM4 (P50) pAb in paraffin-embedded human testis tissue.

Western blot

Western blot of MCM4 (P50) pAb in extracts from HeLa cells.
Western blot of MCM4 (P50) pAb in extracts from HeLa cells.


Requested From: 
Date Requested: 6/19/2018

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