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Antibodies > MCM2 > LS-C352500
 
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Catalog Number Size Price
LS-C352500-100 100 µl (1 mg/ml) $299 
MCM2 Antibody - Immunohistochemical analysis of MCM2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MCM2 Antibody - Immunofluorescent analysis of MCM2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MCM2 Antibody - Western blot analysis of MCM2 expression in HeLa (A); Jurkat (B); HeLa (C) whole cell lysates.
MCM2 Antibody - Immunohistochemical analysis of MCM2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MCM2 Antibody - Immunofluorescent analysis of MCM2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MCM2 Antibody - Western blot analysis of MCM2 expression in HeLa (A); Jurkat (B); HeLa (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human MCM2 Antibody (N‑Terminus, IHC, IF, WB) LS‑C352500

Polyclonal Rabbit anti‑Human MCM2 Antibody (N‑Terminus, IHC, IF, WB) LS‑C352500

Submit Review LSBio Guarantee
Antibody:
MCM2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352500-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MCM2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MCM2 antibody LS-C352500 is an unconjugated rabbit polyclonal antibody to human MCM2 (N-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human MCM2
Synonyms
MCM2 | BM28 | Cell devision cycle-like 1 | D3S3194 | Cdc19 | Cyclin-like 1 | KIAA0030 | Nuclear protein BM28 | CDCL1 | MITOTIN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human MCM2.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of MCM2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM2
P49736 NM_004526 NP_004517.2

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Species: Human, Mouse, Rat, S. cerevisiae
Applications: IHC, IHC - Paraffin, Western blot, ELISA
MCM2 Antibody - Detection of Human Phospho MCM2 (Ser 41) by Immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) or CIP phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser 41) used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody 1:100.
MCM2 Antibody (phospho‑Ser41) LS‑C285953
Species: Human, Mouse
Applications: IHC
MCM2 Antibody - IHC of paraffin-embedded colon cancer tissues using MCM2 mouse monoclonal antibody with DAB staining.
MCM2 Antibody (clone 1E7) LS‑C169438
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
MCM2 Antibody - Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
PathPlus™ MCM2 Antibody (aa1‑50) LS‑B7044
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
MCM2 Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
MCM2 Antibody (clone OTI1G4, Carrier‑free) LS‑C790619
Species: Human
Applications: IHC, Western blot
MCM2 Antibody - Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) ( Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
MCM2 Antibody (phospho‑Ser40/41) LS‑C287647
Species: Human
Applications: IHC, Western blot, Flow Cytometry
See all MCM2 Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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