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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MCM2 Antibody (clone 2546C1a) LS‑C184630
MCM2 antibody LS-C184630 is an unconjugated mouse monoclonal antibody to human MCM2. Validated for DB, ICC and WB.
Catalog
Size
Price
LS-C184630-50
50 µg (0.1 mg/ml)
Unavailable

Popular MCM2 Products

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Detection of Human Phospho MCM2 (Ser 41) by Immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) or CIP phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser 41) used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody 1:100.
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Detection of Human and Mouse MCM2 (S53) Immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM2 (S53) No. Lot4) used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
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Detection of Human MCM2 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.
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Product Description

MCM2 antibody LS-C184630 is an unconjugated mouse monoclonal antibody to human MCM2. Validated for DB, ICC and WB.
About MCM2
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. P49736 NM_004526 NP_004517.2

MCM2 Antibody, BM28 Antibody, Cell devision cycle-like 1 Antibody, D3S3194 Antibody, Cdc19 Antibody, Cyclin-like 1 Antibody, KIAA0030 Antibody, Nuclear protein BM28 Antibody, CDCL1 Antibody, MITOTIN Antibody


Specifications

Target
Human MCM2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2546C1a]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot (0.2 - 2 µg/ml)
  • Dot Blot
Immunogen
MCM2 antibody was raised against recombinant Human Minichromosome Maintenance Complex Component 2 (Mcm2).
Specificity
Human MCM2
Presentation
PBS, pH 7.4, 1% BSA, 0.05% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using MCM2 antibody. Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.


Requested From: 
Date Requested: 6/22/2018

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