Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MCL1 / MCL 1 antibody was raised against recombinant human Mcl-1 protein corresponding to amino acid 1-327.
Recognizes human Mcl-1 (Myeloid cell leukemia-1) (MW 42kD).
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1:100-1:200. Immunoprecipitation: 10 ul/mg of cell lysate. Immunohistochemistry: (with cryostat or formalin-fixed paraffin embedded sections). Staining of formalin fixed paraffin embedded tissues requires boiling the tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Western Blot: ML-1 or Raji cells.
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.