LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
MCL1 / MCL 1 Antibody (clone 10F6) LS‑C115080
MCL 1 antibody LS-C115080 is an unconjugated mouse monoclonal antibody to human MCL 1 (MCL1). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C115080-100
100 µl (0.59 mg/ml)
Unavailable

Popular MCL1 / MCL 1 Products

Anti-MCL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B586 concentration 2 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
IHC of paraffin-embedded human lymph node showing cytoplasmic localization using MCL-1 antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-MCL1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5215 dilution 1:25.
Species: Human
Applications: IHC, IHC - Paraffin
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10210 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

MCL 1 antibody LS-C115080 is an unconjugated mouse monoclonal antibody to human MCL 1 (MCL1). Validated for Flow, IF, IHC and WB.
About MCL1 / MCL 1
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Q07820 NM_021960 NP_068779.1

MCL1 Antibody, BCL2L3 Antibody, Bcl-2-like protein 3 Antibody, Bcl-2-related protein EAT/mcl1 Antibody, Bcl2-L-3 Antibody, MCL1S Antibody, MCL1L Antibody, Myeloid cell leukemia ES Antibody, MCL 1 Antibody, MCL1-ES Antibody, TM Antibody, EAT Antibody, Mcl-1 Antibody


Specifications

Target
Human MCL1 / MCL 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [10F6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCL1 / MCL 1 antibody was raised against full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Specificity
Human MCL 1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/23/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number