MCL1, BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, Bcl2-L-3, MCL1S, MCL1L, Myeloid cell leukemia ES, MCL 1, MCL1-ES, TM, EAT, Mcl-1
(tested or 100% immunogen sequence identity)
MCL1 / MCL 1 antibody was raised against synthetic peptide corresponding to amino acids 121-139 of the Mcl-1 protein.
Recognizes the human Mcl-1 apoptosis related protein, a member of the Bcl-2 family. Western Blotting experiments show a band of approx 38kD corresponding to intact Mcl-1.
Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (paraffin): 1:200-1:1000. Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal results may be obtained using Bouin's fixative and heat mediated antigen retrieval although neutral buffered formalin may also be used. Western Blot: 1:500-1:1000. Positive control: Human Tonsil.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.