MCL 1 antibody LS-C8946 is an unconjugated mouse monoclonal antibody to human MCL 1 (MCL1). Validated for IP and WB.
Human MCL1 / MCL 1
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 - 2 µg/ml)
MCL1 / MCL 1 antibody was raised against recombinant human Mcl-1 protein corresponding to aa1-327.
Recognizes human Mcl-1 at 42kD.
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1:25 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Raji cells. Spleen or tonsil.
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol, 0.2% BSA
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.