Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein G purified
Western blot (1 - 2 µg/ml)
IHC - Paraffin
MCL1 / MCL 1 antibody was raised against recombinant human Mcl-1 protein corresponding to aa1-327.
Recognizes human Mcl-1 at 42kD.
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1:25 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Raji cells. Spleen or tonsil.
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.