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Antibodies > mCherry > LS-C204825
 
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Catalog Number Size Price
LS-C204825-100 100 µl (1 mg/ml) $376 
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
mCherry Antibody - Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
mCherry Antibody - Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
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Polyclonal Chicken anti‑Discosoma mCherry Antibody (IF, WB) LS‑C204825

Polyclonal Chicken anti‑Discosoma mCherry Antibody (IF, WB) LS‑C204825

(4.2) Rating
Submit Review (3) Publication(s) LSBio Guarantee
Antibody:
mCherry Chicken anti-Discosoma Polyclonal Antibody
Application:
ICC, IF, WB
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204825-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
mCherry Chicken anti-Discosoma Polyclonal Antibody
Application:
ICC, IF, WB
Format:
Unconjugated, Unmodified

Specifications

Description
MCherry antibody LS-C204825 is an unconjugated chicken polyclonal antibody to mCherry from discosoma. Validated for ICC, IF and WB. Cited in 3 publications.
(3) Publication(s)
Target
Discosoma mCherry
Host
Chicken
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
IgY fraction
Modifications
Unmodified
Immunogen
Recombinant full length His tagged mCherry purified from E. coli.
Specificity
mCherry
Applications
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:2000)
Usage
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.
Presentation
5 mM Sodium Azide
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

mCherry Antibody for ICC, IF/Immunofluorescence, WB/Western LS-C204825 has an LSBio Rating of
(4.2) Rating
Publications (4.2)

Publications (3)

Chemogenetic Activation of an Extinction Neural Circuit Reduces Cue-Induced Reinstatement of Cocaine Seeking. Augur IF, Wyckoff AR, Aston-Jones G, Kalivas PW, Peters J. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2016 36:10174-80. (IHC; Rat)
Full Text Article PubMed: 27683912 PMC: PMC5039261
Selection and Characterization of Rupintrivir-Resistant Norwalk Virus Replicon Cells In Vitro. Kitano M, Hosmillo M, Emmott E, Lu J, Goodfellow I. Antimicrobial agents and chemotherapy. 2018 May;62:e00201-18. (Discosoma)
Full Text Article PubMed: 29530860 PMC: PMC5923142
Behavioral and slice electrophysiological assessment of DREADD ligand, deschloroclozapine (DCZ) in rats. Todd B Nentwig , J Daniel Obray , Dylan T Vaughan, L Judson Chandler. Scientific reports. 2022 April;12:6595.
Full Text Article PubMed: 35449195 PMC: PMC9023443
Submit a Publication for LS-C204825

Customer Reviews (0)

Submit a review for LS-C204825

Featured Products

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mCherry Antibody LS‑C204207
Species: Discosoma
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
mCherry Antibody LS‑C204826
Species: Discosoma
Applications: ICC, Immunofluorescence, Western blot
mCherry Antibody - Shows HEK293 cells transfected with mCherry and visualized in red. The cells were stained with mCherry antibody in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the mCherry antibody antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
mCherry Antibody (clone 1C51) LS‑C204827
Species: Discosoma
Applications: ICC, Immunofluorescence, Western blot
mCherry Antibody - HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.2ug, 0.4ug, 0.8ug, 1.6ug of lysates. (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit mCherry polyclonal antibody at 1:10000.
mCherry Antibody LS‑C784928
Species:
Applications: Western blot
KCNA1 / Kv1.1 Antibody - Detection limit for recombinant GST tagged KCNA1 is 0.3 ng/ml as a capture antibody.
KCNA1 / Kv1.1 Antibody (clone 2D8) LS‑C197568
Species: Human
Applications: Western blot, ELISA
Product
Human HMG1 / HMGB1 Protein (Recombinant 6His, C-terminus) - LS-G145709
Source: E. coli
Tag: 6His, C-terminus
See all mCherry Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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