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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3628-50 | 50 µg (0.25 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (Tyr641, IHC, WB) LS‑B3628
IHC‑plus™ Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (Tyr641, IHC, WB) LS‑B3628
Note: This antibody replaces LS-C97242
Antibody:
MCAM / CD146 Rabbit anti-Human Polyclonal (Tyr641) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MCAM / CD146 Rabbit anti-Human Polyclonal (Tyr641) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD146 antibody LS-B3628 is an unconjugated rabbit polyclonal antibody to human CD146 (MCAM) (Tyr641). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Epitope
Tyr641
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3628 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3628 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MCAM / CD146
Validation
Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MCAM / CD146 Antibody (Tyr641) for IHC, WB/Western LS-B3628 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Rat
Applications:
Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
Western blot, ELISA
Species:
Human, Mouse, Dog, Rabbit
Applications:
IHC, ICC, Immunoprecipitation, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
