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Anti-MBD3 Antibody (aa215-230, clone 106B691) IHC-plus™ LS-B2053

Note: This antibody replaces LS-C565

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MBD3 Antibodies

Anti-MBD3 Antibody (aa215-230, clone 106B691) IHC-plus™ LS-B2053
Mouse Monoclonal [clone 106B691] (IgG1) to Human MBD3
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MBD3 Antibody (aa220-234, clone 3A3) LS-C193584
Mouse Monoclonal [clone 3A3] (IgG1) to Human MBD3
Human, Monkey, Mouse, Rat, Hamster
Western blot
Unconjugated
Anti-MBD3 Antibody (aa1-290, clone ABM14A8) IHC-plus™ LS-B13489
Mouse Monoclonal [clone ABM14A8] (IgG1,k) to Human MBD3
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 106B691] (IgG1) to Human MBD3
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated

Details

Human MBD3
Mouse
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Hamster, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
IgG1 Monoclonal [106B691]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

MBD3 antibody was raised against amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%); Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%); Salmon (88%).
aa215-230
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
Immunohistochemistry: LS-B2053 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2053 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide. Sourced in TCS.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MBD3

O95983 NM_003926 NP_003917.1

MBD3 Antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA.

Immunohistochemistry

Immunohistochemistry
Anti-MBD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2053 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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