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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2053-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human MBD3 Antibody (clone 106B691, aa215‑230, IHC, WB) LS‑B2053
IHC‑plus™ Monoclonal Mouse anti‑Human MBD3 Antibody (clone 106B691, aa215‑230, IHC, WB) LS‑B2053
Note: This antibody replaces LS-C565
Antibody:
MBD3 Mouse anti-Human Monoclonal (aa215-230) (106B691) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MBD3 Mouse anti-Human Monoclonal (aa215-230) (106B691) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
MBD3 antibody LS-B2053 is an unconjugated mouse monoclonal antibody to MBD3 (aa215-230) from human. It is reactive with human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Hamster, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
106B691
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%); Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%); Salmon (88%).
Epitope
aa215-230
Specificity
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2053 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2053 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MBD3
Validation
Anti-MBD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MBD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MBD3 Antibody (aa215-230, clone 106B691) for IHC, WB/Western LS-B2053 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Source:
E. coli
Tag:
His
Reactivity:
Human
Range:
46.88-3000 pg/ml
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
