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Anti-MBD1 Antibody (C-Terminus) IHC-plus™ LS-B1545

Note: This antibody replaces LS-C30510
Catalog Size Price
LS-B1545-50 50 µg Unavailable

Related Products

39 MBD1 Antibodies

Most Popular MBD1 Antibodies

Anti-MBD1 Antibody (C-Terminus) LS-C31263
Rabbit Polyclonal (IgG) to Human MBD1
Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot
Western blot Image
Anti-MBD1 Antibody LS-C672572
Rabbit Polyclonal (IgG) to Human MBD1
Immunohistochemistry - Paraffin Image
Anti-MBD1 Antibody (Biotin) LS-C672573
Rabbit Polyclonal (IgG) to Human MBD1
Biotin Conjugated
Anti-MBD1 Antibody (FITC) LS-C672575
Rabbit Polyclonal (IgG) to Human MBD1
FITC Conjugated
Anti-MBD1 Antibody (HRP) LS-C675087
Rabbit Polyclonal (IgG) to Human MBD1
HRP Conjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human MBD1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)


Human MBD1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified


  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MBD1 antibody was raised against synthetic peptide from C-Terminus of human MBD1 (NP_056669). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Tamarin, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Mouse, Rat (85%).
LS-E11187 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1545. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human MBD1
Immunohistochemistry: LS-B1545 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1545 was determined to be 10 ug/ml.


Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MBD1

Q9UIS9 NM_015846 NP_056671.2

MBD1 Antibody, CXXC3 Antibody, RFT Antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.


Anti-MBD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1545 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
MBD1 antibody LS-B1545 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/22/2018

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