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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPRE2 / EB2 Antibody (clone 3C7) LS‑C172982
EB2 antibody LS-C172982 is an unconjugated mouse monoclonal antibody to EB2 (MAPRE2) from human, rat, dog and other species. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C172982-100
100 µl (1 mg/ml)
Unavailable

Popular MAPRE2 / EB2 Products

Immunofluorescence of monoclonal antibody to MAPRE2 on HeLa cell (antibody concentration 35 ug/ml).
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAPRE2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-MAPRE2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-MAPRE2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-MAPRE2 / EB2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9959 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

EB2 antibody LS-C172982 is an unconjugated mouse monoclonal antibody to EB2 (MAPRE2) from human, rat, dog and other species. Validated for Flow, IF and WB.
About MAPRE2 / EB2
MAPRE2 / EB2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. Q15555 NM_014268 NP_055083.1

MAPRE2 Antibody, APC-binding protein EB2 Antibody, APC-binding protein EB1 Antibody, EB1 Antibody, EB2 Antibody, End-binding protein 2 Antibody


Specifications

Target
Human MAPRE2 / EB2
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [3C7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Immunogen
MAPRE2 / EB2 antibody was raised against full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
Specificity
Human MAPRE2 / EB2
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.
Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.
Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.
Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.
Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPRE2 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/22/2018

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