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Anti-MAPKAPK5 / PRAK Antibody (aa192-206) IHC-plus™ LS-B9569

Note: This antibody replaces LS-C186551

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPKAPK5 / PRAK Antibodies

Anti-MAPKAPK5 / PRAK Antibody LS-C41490
Mouse Monoclonal (IgG1) to Human MAPKAPK5 / PRAK
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C381421
Rabbit Polyclonal (IgG) to Human MAPKAPK5 / PRAK
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Anti-MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C291607
Rabbit Polyclonal (IgG) to Human MAPKAPK5 / PRAK
Human, Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MAPKAPK5 / PRAK
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MAPKAPK5 / PRAK
Goat
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (1:64000)

Specificity and Use

MAPKAPK5 / PRAK antibody was raised against synthetic peptide C-PQVLEAQRRHQKEKS from an internal region of human MAPKAPK5 / PRAK (NP_003659.2; NP_620777.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Lizard, Stickleback, Medaka, Pufferfish, Zebrafish (100%); Turkey, Platypus, Xenopus (93%); Sea squirt (80%).
LS-E28034 - Lyophilized - 100 µg - $145.00
aa192-206
Human MAPKAPK5 / PRAK
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11927 or EB11928. Western blots of the IP were labeled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kD of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MAPKAPK5 / PRAK

Q8IW41 NM_003668 NP_003659.2

MAPKAPK5 Antibody, MAPKAP kinase 5 Antibody, MAPKAP-K5 Antibody, MK5 Antibody, p38-regulated/activated kinase Antibody, MAPKAPK-5 Antibody, PRAK Antibody, MK-5 Antibody, p38-regulated protein kinase Antibody

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53.

Immunohistochemistry

Immunohistochemistry
Anti-MAPKAPK5 / PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9569 concentration 5 ug/ml.

Western blot

Western blot
MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with EB11927 (0.5 ug/ml) in right panel and with (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.

Western blot

Western blot
MAPKAPK5 antibody EB11927 and (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
 

Requested From: United States
Date Requested: 12/10/2016

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