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| Catalog Number | Size | Price |
|---|---|---|
| LS-C186541-100 | 100 µg (0.5 mg/ml) | $503 |
1 of 2
2 of 2
Polyclonal Goat anti‑Human MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa44‑55, WB) LS‑C186541
Polyclonal Goat anti‑Human MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa44‑55, WB) LS‑C186541
Antibody:
MAPKAPK2 / MAPKAP Kinase 2 Goat anti-Human Polyclonal (aa44-55) Antibody
Application:
WB, IP, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MAPKAPK2 / MAPKAP Kinase 2 Goat anti-Human Polyclonal (aa44-55) Antibody
Application:
WB, IP, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
MAPKAP Kinase 2 antibody LS-C186541 is an unconjugated goat polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2) (aa44-55) from human. It is reactive with human, mouse and monkey. Validated for IP, Peptide-ELISA and WB.
Target
Human MAPKAPK2 / MAPKAP Kinase 2
Synonyms
MAPKAPK2 | MK-2 | MAP KAP Kinase 2 | MAPKAP kinase 2 | MK2 | MAPKAP-K2 | MAPKAPK-2
Host
Goat
Reactivity
Human, Monkey, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-PQFHVKSGLQIK, from the internal region of the protein sequence according to NP_004750.1NP_116584.2.
Epitope
aa44-55
Specificity
Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation (1.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 ug/ml. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 ug EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MAPKAPK2 / MAPKAP Kinase 2
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot, Immunoprecipitation, ELISA, Dot Blot
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Rabbit, Chicken, Zebrafish
Applications:
IHC, Western blot
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot
![MAPKAPK2 / MAPKAP Kinase 2 Antibody - Anti-MAPKAP Kinase 2 Polyclonal Antibody - Western Blot. Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kD protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/mapkapk2-mapkap-kinase-2-antibody-ls-c18843/62803_955632.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
