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Anti-MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa44-55) LS-C186541

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

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Most Popular MAPKAPK2 / MAPKAP Kinase 2 Antibodies

Anti-MAPKAPK2 / MAPKAP Kinase 2 Antibody (phospho-Thr334) LS-C124052
Rabbit Polyclonal (IgG) to Human MAPKAPK2 / MAPKAP Kinase 2
Human, Monkey, Mouse, Rat, Bovine, Dog, Rabbit, Chicken, Zebrafish
IHC, Western blot
Unconjugated
Anti-MAPKAPK2 / MAPKAP Kinase 2 Antibody (clone 3B8) LS-C133110
Mouse Monoclonal [clone 3B8] (IgG2a,k) to Human MAPKAPK2 / MAPKAP Kinase 2
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image
Anti-MAPKAPK2 / MAPKAP Kinase 2 Antibody (Ser272, FITC) LS-C279205
Rabbit Polyclonal (IgG) to Human MAPKAPK2 / MAPKAP Kinase 2
Human
IHC, Western blot, Immunoprecipitation, ELISA, Dot Blot
FITC Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MAPKAPK2 / MAPKAP Kinase 2
Human, Monkey, Mouse
Western blot, ELISA
Unconjugated

Details

Human MAPKAPK2 / MAPKAP Kinase 2
Goat
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Rat, Guinea pig, Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.5 - 2 µg/ml)
  • ELISA (1:32000)

Specificity and Use

MAPKAPK2 / MAPKAP Kinase 2 antibody was raised against synthetic peptide C-PQFHVKSGLQIK from an internal region of human MAPKAPK2 / MK2 (NP_004750.1; NP_116584.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey (100%); Galago, Mouse, Rat, Panda, Rabbit, Pig, Guinea pig (92%); Bovine, Opossum (83%).
LS-E28036 - Lyophilized - 100 µg - $145.00
aa44-55
Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 ug/ml. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 ug EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MAPKAPK2 / MAPKAP Kinase 2

P49137 NM_004759 NP_004750.1

MAPKAPK2 Antibody, MK-2 Antibody, MAP KAP Kinase 2 Antibody, MAPKAP kinase 2 Antibody, MK2 Antibody, MAPKAP-K2 Antibody, MAPKAPK-2 Antibody

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue.

Western blot

Western blot
MAPKAPK2 antibody EB11929 and (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-MK2.

Immunoprecipitation

Immunoprecipitation
MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.

Requested From: United States
Date Requested: 12/3/2016

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