Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1C1-3A8] (IgG1,k) to Human MAPK9 / JNK2
IHC - Paraffin, Western blot, ELISA
Human MAPK9 / JNK2
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1C1-3A8]
Protein A purified
IHC - Paraffin (5 µg/ml)
Western blot (1:500 - 1:1000)
Specificity and Use
MAPK9 / JNK2 antibody was raised against mAPK9 (AAH32539, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysated and the recombinant protein used as the immunogen.
PBS, pH 7.4. Sourced in Ascites.
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2.
Anti-MAPK9 / JNK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6137 concentration 5 ug/ml.
Western blot of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody LS-B6137
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MAPK9 is approximately 3 ng/ml as a capture antibody.
Western blot of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody LS-B6137. GAPDH ( 36.1 kD ) used as specific.
Requested From: United States
Date Requested: 2/23/2017