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Anti-MAPK8 / JNK1 / JNK Antibody (aa5-300) LS-C185392

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.4 mg/ml $335

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MAPK8 / JNK1 / JNK Antibody LS-C185392 Images

[click image for a larger image and more details]
Immunocytochemistry
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using JNK1...
Western blot
Sample (30 ug of whole cell lysate).
Western blot
Sample (50 ug of whole cell lysate).

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Rabbit Polyclonal (IgG) to Human MAPK8 / JNK1 / JNK
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human MAPK8 / JNK1 / JNK
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Bovine, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

MAPK8 / JNK1 / JNK antibody was raised against recombinant fragment corresponding to a region within amino acids 5 and 300 of JNK1 (SwissProt P45983). Percent identity by BLAST analysis: Human (100%); Bovine (96%); Xenopus (94%); Zebrafish (87%).
aa5-300
Human MAPK8 / JNK1 / JNK

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About MAPK8 / JNK1 / JNK

P45983 NM_139049 NP_620637.1

MAPK8 Antibody, JNK Antibody, JNK1 Antibody, JNK1A2 Antibody, JNK-46 Antibody, MAP kinase 8 Antibody, PRKM8 Antibody, SAPK1 Antibody, SAPK1c Antibody, MAPK 8 Antibody, C-Jun N-terminal kinase 1 Antibody, JNK21B1/2 Antibody, JUN N-terminal kinase Antibody

JNK1, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK1 is phosphorylated by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK1 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4. At least four alternatively spliced transcript variants encoding distinct isoforms, 384 and 427 amino acids, have been identified.

Immunocytochemistry

Immunocytochemistry
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. MAPK8 / JNK1 / JNK antibody diluted at 1:1000.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. MAPK8 / JNK1 / JNK antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 6/30/2015

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