JNK1 antibody LS-C16104 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from human, mouse, rat and other species. Validated for IP and WB.
Human MAPK8 / JNK1
Human, Mouse, Rat, Chicken, Drosophila, C. elegans
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (1 µg/ml)
- Immunoprecipitation (1 µg/ml)
- (applications tested for the base form of this product only)
MAPK8 / JNK1 antibody was raised against synthetic peptide corresponding to aa181-190 [(MM[pT]P[pY]VVTRY-C)] of human/mouse/rat JNK1 (MAPK 8) and human/mouse/rat JNK2 (MAPK 9). Sequence also matches aa219-229 of human/mouse/rat JNK3.
Recognizes phosphorylated JNK, Mr 46kD and 55kD. Species cross-reactivity: Human, rat, mouse. Expected to cross-react with Drosophila, C. elegans and chicken based on sequence homology.
Suitable for use in Immunoprecipitation/Western Blot. Immunoprecipitation/Western Blot: 1 ug/ml. Anti-pan JNK/SAPK1 was used to Immunoprecipitate JNK from lysates of ultraviolet light-treated RAW 264 macrophage. Phosphorylated JNK (T183/Y185) was detected. Phosphorylated JNK was not detected in non-treated lysates.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.