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Anti-MAPK8 / JNK1 Antibody (N-Terminus) IHC-plus™ LS-B3403

Note: This antibody replaces LS-C84063

Ordering

Wt. Vol. Conc. Price
50 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPK8 / JNK1 Antibodies

Anti-MAPK8 / JNK1 Antibody (phospho-Thr183/Tyr185) LS-C84066
Rabbit Polyclonal to Human MAPK8 / JNK1
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila
Western blot, ELISA
Unconjugated
Anti-MAPK8 / JNK1 Antibody (N-Terminus) IHC-plus™ LS-B3403
Rabbit Polyclonal to Human MAPK8 / JNK1
Human, Monkey, Mouse, Rat, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MAPK8 / JNK1 Antibody (clone 2F3) IHC-plus™ LS-B6390
Mouse Monoclonal [clone 2F3] (IgG2a,k) to Human MAPK8 / JNK1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MAPK8 / JNK1
Human, Monkey, Mouse, Rat, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Human MAPK8 / JNK1
Rabbit
Human, Monkey, Mouse, Rat, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

MAPK8 / JNK1 antibody was raised against synthetic peptide corresponding to the N- terminal portion of human JNK1.
N-Terminus
Reacts specifically with JNK1. The antibody was evaluated by western blot, dot blot and ELISA. By western blot, an immunoreactive bands around 46 kD was observed in the NIH/3T3 whole cell lysate. Western blot:0.5-2 ug/ml IP*:2-5 ug/extract from 107 Cells IHC*:2-5 ug/ml *: Recommended but not tested. This product is for vitro Research Use Only. 75kDWB: 3T3 whole cell lysate 50was probed with anti-JNK 25(NT-1) antibody at 1:200 dilution.
Immunohistochemistry: LS-B3403 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3403 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.05% sodium azide
Store at 4°C. Do not freeze.
For research use only.

About MAPK8 / JNK1

P45983 NM_139049 NP_620637.1

MAPK8 Antibody, JNK Antibody, JNK1 Antibody, JNK1A2 Antibody, JNK-46 Antibody, MAP kinase 8 Antibody, PRKM8 Antibody, SAPK1 Antibody, SAPK1c Antibody, MAPK 8 Antibody, C-Jun N-terminal kinase 1 Antibody, JNK21B1/2 Antibody, JUN N-terminal kinase Antibody

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1.

Immunohistochemistry

Immunohistochemistry
Anti-MAPK8 / JNK1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3403 concentration 5 ug/ml.

Western blot

Western blot
3T3 whole cell lysate was probed with anti-JNK (NT-1) antibody at 1:200 dilution.

Requested From: United States
Date Requested: 12/11/2016

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