Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3H2] (IgG2a,k) to Human MAPK8 / JNK1
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Rabbit Polyclonal (IgG) to Human MAPK8 / JNK1
ICC, Western blot
Human MAPK8 / JNK1
Human, Mouse (tested or 100% immunogen sequence identity)
Bovine, Xenopus (at least 90% immunogen sequence identity)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
MAPK8 / JNK1 antibody was raised against recombinant fragment corresponding to a region within amino acids 5 and 300 of JNK1 (SwissProt P45983). Percent identity by BLAST analysis: Human (100%); Bovine (96%); Xenopus (94%); Zebrafish (87%).
Human MAPK8 / JNK1 / JNK
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. MAPK8 / JNK1 / JNK antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. MAPK8 / JNK1 / JNK antibody diluted at 1:1000.