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Anti-MAPK7 / ERK5 Antibody (aa1-432) LS-C150109

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPK7 / ERK5 Antibodies

Anti-MAPK7 / ERK5 Antibody (aa776-805) LS-C99955
Rabbit Polyclonal to Human MAPK7 / ERK5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MAPK7 / ERK5 Antibody (C-Terminus, Biotin) LS-C219117
Rabbit Polyclonal (IgG) to Human MAPK7 / ERK5
Human
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human MAPK7 / ERK5
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human MAPK7 / ERK5
Goat
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

MAPK7 / ERK5 antibody was raised against e. coli-derived recombinant human ERK5/BMK1 Met1-Glu432 Accession # Q13164.
aa1-432
Detects human and mouse ERK5/BMK1 in Western blots.
Western Blot, 1 µg/ml. Immunocytochemistry, 5-15 µg/ml, Immersion fixed human peripheral blood mononuclear cells.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About MAPK7 / ERK5

Q13164 NM_002749 NP_002740.2

MAPK7 Antibody, Big MAP kinase 1 Antibody, BMK-1 Antibody, Erk4 Antibody, ERK5 Antibody, MAPK 7 Antibody, PRKM7 Antibody, BMK1 Antibody, BMK1 kinase Antibody, ERK-5 Antibody, MAP kinase 7 Antibody

ERK5, a MAPK type protein kinase, mediates growth factor stimulated cell proliferation and muscle cell differentiation. ERK5 differs from other MAP kinases in that it contains a unique loop-12 region within the protein kinase domain which is followed by an extensive C-terminal tail with sequences indicating that it may be targeted to the cytoskeleton. ERK5 is phosphorylated and activated by MEK5 in response to osmotic shock, growth factors and oxidant stress.

Western blot

Western blot
Detection of Human/Mouse ERK5/BMK1 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ERK5/BMK1 at approximately 115 kD (as indicated). This experiment was conducted under reducing conditions.
 

Requested From: United States
Date Requested: 12/11/2016

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