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Anti-MAPK6 / ERK3 Antibody (clone 1G6) LS-C197995

Catalog Size Price
LS-C197995-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1G6] (IgG2a,k) to Human MAPK6 / ERK3
Human
Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated

Details

Human MAPK6 / ERK3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1G6]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

MAPK6 / ERK3 antibody was raised against mAPK6 (AAH35492, aa612-721 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MAPK6 / ERK3
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About MAPK6 / ERK3

Q16659 NM_002748 NP_002739.1

MAPK6 Antibody, ERK-3 Antibody, MAP kinase 6 Antibody, MAPK 6 Antibody, p97-MAPK Antibody, PRKM6 Antibody, MAP kinase isoform p97 Antibody, ERK3 Antibody, HsT17250 Antibody, p97MAPK Antibody

ERK3, a MAPK type protein kinase, has been associated with the ERK MAPKs based on similarity of the catalytic domain. Compared to ERK1/2, ERK 3 lacks the activating tyrosine phosphorylation site and contains only a serine which is phosphorylated by MEK. ERK3 is primarily localized to the nucleus where it is believed to phosphorylate substrates in response to cell stress and damage.

Immunofluorescence

Immunofluorescence of monoclonal antibody to MAPK6 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to MAPK6 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MAPK6 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MAPK6 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: 
Date Requested: 5/26/2018

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