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Anti-MAPK6 / ERK3 Antibody (aa395-444) LS-C135383

Catalog Size Price
LS-C135383-50 50 µg Unavailable

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46 MAPK6 / ERK3 Antibodies

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Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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100% Guaranteed
Rabbit Polyclonal to Human MAPK6 / ERK3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated
MAPK6 antibody LS-C135383 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Human MAPK6 / ERK3
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MAPK6 / ERK3 antibody was raised against synthetic peptide located between aa395-444 of human MAPK6 (Q16659, NP_002739). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%); Elephant (92%); Stickleback (85%).
LS-E20487 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135383. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa395-444
Human MAPK6
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MAPK6 / ERK3

Q16659 NM_002748 NP_002739.1

MAPK6 Antibody, ERK-3 Antibody, MAP kinase 6 Antibody, MAPK 6 Antibody, p97-MAPK Antibody, PRKM6 Antibody, MAP kinase isoform p97 Antibody, ERK3 Antibody, HsT17250 Antibody, p97MAPK Antibody

ERK3, a MAPK type protein kinase, has been associated with the ERK MAPKs based on similarity of the catalytic domain. Compared to ERK1/2, ERK 3 lacks the activating tyrosine phosphorylation site and contains only a serine which is phosphorylated by MEK. ERK3 is primarily localized to the nucleus where it is believed to phosphorylate substrates in response to cell stress and damage.
(More About MAPK6 / ERK3)

Western blot

MAPK6 antibody LS-C135383 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAPK6 antibody LS-C135383 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: 
Date Requested: 4/24/2018
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