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Anti-MAPK6 / ERK3 Antibody (aa395-444) LS-C135383

Catalog	Size	Price
LS-C135383-50	50 µg	Unavailable

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Anti-MAPK6 / ERK3 Antibody (Internal) LS-C354065

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Anti-MAPK6 / ERK3 Antibody (aa430-510) LS-C383407

Antibody:	Rabbit Polyclonal (IgG) to Human MAPK6 / ERK3
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Application:	IHC, Immunofluorescence, Western blot, ELISA
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Anti-MAPK6 / ERK3 Antibody (aa520-721) LS-C407877

Antibody:	Rabbit Polyclonal (IgG) to Human MAPK6 / ERK3
Reactivity:	Human, Mouse, Rat, Dog
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human MAPK6 / ERK3
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human MAPK6 / ERK3
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

MAPK6 / ERK3 antibody was raised against synthetic peptide located between aa395-444 of human MAPK6 (Q16659, NP_002739). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%); Elephant (92%); Stickleback (85%).

Blocking Peptide:

LS-E20487 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135383. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa395-444

Specificity:

Human MAPK6

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About MAPK6 / ERK3

Uniprot: Q16659 NCBI: NM_002748 NP_002739.1

Synonyms: MAPK6 Antibody, ERK-3 Antibody, MAP kinase 6 Antibody, MAPK 6 Antibody, p97-MAPK Antibody, PRKM6 Antibody, MAP kinase isoform p97 Antibody, ERK3 Antibody, HsT17250 Antibody, p97MAPK Antibody

Description: ERK3, a MAPK type protein kinase, has been associated with the ERK MAPKs based on similarity of the catalytic domain. Compared to ERK1/2, ERK 3 lacks the activating tyrosine phosphorylation site and contains only a serine which is phosphorylated by MEK. ERK3 is primarily localized to the nucleus where it is believed to phosphorylate substrates in response to cell stress and damage.

(More About MAPK6 / ERK3)

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Western blot

MAPK6 antibody LS-C135383 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/26/2017

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