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| Catalog Number | Size | Price |
|---|---|---|
| LS-C16358-100 | 100 µg | $842 |
Polyclonal Rabbit anti‑Rat MAPK3 / ERK1 Antibody (C‑Terminus, IHC, WB) LS‑C16358
Polyclonal Rabbit anti‑Rat MAPK3 / ERK1 Antibody (C‑Terminus, IHC, WB) LS‑C16358
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
ERK1 antibody LS-C16358 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (C-Terminus) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB.
Target
Rat MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide (aa 339-353) corresponding to a C-Terminus segment of rat ERK1.
Epitope
C-Terminus
Specificity
Reacts strongly with ERK1 and ERK2. A synthetic peptide, corresponding to a 15 amino acid sequence near the carboxyl terminus (339-353 aa of rat ERK1) was conjugated to carrier protein and used as the immunogen. Reactivity to ERK1 and ERK2 has been confirmed by western blot analysis of human A431 and K562 cells, mouse brain and rat brain. Human, mouse and rat (positive control: human A431 and K562 cells, mouse brain and rat brain cell lysates).
Applications
- IHC (5 - 10 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. ELISA: 0.1-1 ug/ml (to coat: 10 ug/ml). Immunoprecipitation: 2-5 ug. Immunohistochemistry: 5-10 ug/ml. Western blotting: ~1:1000. Positive control: Human A431 and K562 cells, mouse brain and rat brain cell lysates).
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MAPK3 / ERK1
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
