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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK12 / ERK6 / SAPK3 Antibody (aa289‑338) LS‑C135387
SAPK3 antibody LS-C135387 is an unconjugated rabbit polyclonal antibody to SAPK3 (MAPK12 / ERK6) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135387-50
50 µg
Unavailable

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Product Description

SAPK3 antibody LS-C135387 is an unconjugated rabbit polyclonal antibody to SAPK3 (MAPK12 / ERK6) from human, mouse, rat and other species. Validated for WB.
About MAPK12 / ERK6 / SAPK3
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. P53778 NM_002969 NP_002960.2

MAPK12 Antibody, ERK5 Antibody, ERK6 Antibody, ERK-6 Antibody, MAP kinase 12 Antibody, MAPK 12 Antibody, MAP kinase p38 gamma Antibody, SAPK3 Antibody, p38-gamma Antibody, p38GAMMA Antibody, SAPK-3 Antibody, ERK3 Antibody, PRKM12 Antibody


Specifications

Target
Mouse MAPK12 / ERK6 / SAPK3
Host
Rabbit
Reactivity
Mouse, Human, Rat, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Dog, Guinea pig, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa289-338 of mouse Mapk12 (O08911, NP_038899). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Rabbit, Opossum (100%); Marmoset, Dog, Bat, Horse, Pig, Guinea pig (92%); Galago, Panda (85%).
Blocking Peptide
LS-E20543 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135387. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa289-338
Specificity
Mouse MAPK12 / ERK5
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAPK12 / ERK5 antibody LS-C135387 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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