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Anti-MAPK1 / ERK2 / p42 Antibody (clone 4C11C11C4) IHC-plus™ LS-B2159

Note: This antibody replaces LS-C47179

Ordering

Wt. Vol. Conc. Price
50 µg - - $395

LSBio (Direct) LSBio (Direct)
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MAPK1 / ERK2 / p42 Antibody LS-B2159 Images

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Immunohistochemistry
Anti-MAPK1 / ERK antibody IHC of human heart.

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Mouse Monoclonal [clone 4C11C11C4] (IgG2a) to Human MAPK1 / ERK2 / p42
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

Details

Human MAPK1 / ERK2 / p42
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [4C11C11C4]
Affinity purified

Applications

IHC - Paraffin (5 µg/ml), IHC - Frozen (1:500 - 1:2000), Western blot (1:500 - 1:2000), ELISA (1:10000)

Specificity and Use

MAPK1 / ERK2 / p42 antibody was raised against recombinant human MAPK1 / ERK.
Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3).
Immunohistochemistry: LS-B2159 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2159 was determined to be 5 ug/ml.

Packaging

PBS, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About MAPK1 / ERK2 / p42

P28482 NM_002745 NP_002736.3

MAPK1 Antibody, ERK Antibody, ERK-2 Antibody, ERK2 Antibody, MAP kinase 1 Antibody, MAPK 2 Antibody, MAPK2 Antibody, MAP kinase isoform p42 Antibody, MAPK 1 Antibody, p41mapk Antibody, PRKM2 Antibody, MAP kinase 2 Antibody, p41 Antibody, p42-MAPK Antibody, PRKM1 Antibody, ERT1 Antibody, p38 Antibody, p40 Antibody, p42MAPK Antibody, Protein tyrosine kinase ERK2 Antibody

ERK2, a MAPK type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK2 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK2 causing dissociation of the MEK/ERK complex.

Immunohistochemistry

Immunohistochemistry
Anti-MAPK1 / ERK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2159 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 4/24/2015

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