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Anti-MAPK1 / ERK2 Antibody (clone 7E7) LS-C114585

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPK1 / ERK2 Antibodies

Anti-MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182) LS-C16335
Mouse Monoclonal (IgG1) to Human MAPK1 / ERK2
Human, Mouse, Rat
Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-MAPK1 / ERK2 Antibody (phospho-Tyr204) LS-C183959
Rabbit Polyclonal (IgG) to Human MAPK1 / ERK2
Human, Mouse, Rat
IHC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7E7] (IgG1) to Human MAPK1 / ERK2
Human, Monkey, Rat, Dog
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human MAPK1 / ERK2
Mouse
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [7E7]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation

Specificity and Use

MAPK1 / ERK2 antibody was raised against protein expressed in 293T cell transfected with human MAPK1 expression vector.
Human MAPK1 / ERK2
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MAPK1 / ERK2

P28482 NM_002745 NP_002736.3

MAPK1 Antibody, ERK Antibody, ERK-2 Antibody, ERK2 Antibody, MAP kinase 1 Antibody, MAPK 2 Antibody, MAPK2 Antibody, MAP kinase isoform p42 Antibody, MAPK 1 Antibody, p41mapk Antibody, PRKM2 Antibody, MAP kinase 2 Antibody, p41 Antibody, p42-MAPK Antibody, PRKM1 Antibody, ERT1 Antibody, p38 Antibody, p40 Antibody, p42MAPK Antibody, Protein tyrosine kinase ERK2 Antibody

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of COS7 cells using anti-MAPK1 mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-MAPK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
 

Requested From: United States
Date Requested: 12/4/2016

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