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Catalog Number Size Price
LS-C114585-100 100 µl (0.85 mg/ml) $434 
MAPK1 / ERK2 Antibody - Immunofluorescent staining of COS7 cells using anti-MAPK1 mouse monoclonal antibody.
MAPK1 / ERK2 Antibody - Anti-MAPK1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1.
MAPK1 / ERK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
MAPK1 / ERK2 Antibody - Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAPK1 / ERK2 Antibody - Immunofluorescent staining of COS7 cells using anti-MAPK1 mouse monoclonal antibody.
MAPK1 / ERK2 Antibody - Anti-MAPK1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1.
MAPK1 / ERK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
MAPK1 / ERK2 Antibody - Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone OTI7E7, IF, WB) LS‑C114585

Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone OTI7E7, IF, WB) LS‑C114585

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (OTI7E7) Antibody
Application:
IF, WB, IP
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$434
LS-C114585-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (OTI7E7) Antibody
Application:
IF, WB, IP
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ERK2 antibody LS-C114585 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) from human. It is reactive with human, rat, dog and other species. Validated for IF, IP and WB.
Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI7E7
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Carrier-free.
Immunogen
Protein expressed in 293T cell transfected with human MAPK1 expression vector.
Specificity
Human MAPK1 / ERK2
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024