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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2159-50 | 50 µg | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 4C11C11C4, IHC, WB) LS‑B2159
IHC‑plus™ Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 4C11C11C4, IHC, WB) LS‑B2159
Note: This antibody replaces LS-C47179
Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (4C11C11C4) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (4C11C11C4) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ERK2 antibody LS-B2159 is an unconjugated mouse monoclonal antibody to human ERK2 (MAPK1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
4C11C11C4
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant human MAPK1 / ERK.
Specificity
Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen (1:500 - 1:2000)
- Western blot (1:500 - 1:2000)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2159 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2159 was determined to be 5 ug/ml.
Presentation
PBS, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MAPK1 / ERK2
Validation
Anti-MAPK1 / ERK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MAPK1 / ERK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MAPK1 / ERK2 Antibody (clone 4C11C11C4) for IHC, WB/Western, ELISA LS-B2159 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Mouse, Human, Rat
Applications:
Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Drosophila
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
