Human MAPK1 / ERK2

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein.  The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein.  The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.

This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources. Reactivity to rat tissues is also anticipated due to high sequence homology. Reactivity against homologues from other sources is not known.

• Western blot (1:1000 - 1:,2000)
• Immunoprecipitation
• ELISA (1:2000 - 1:10000)

This antiserum was tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kD in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract. p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf. The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent: U937, HeLa, NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P28482 NCBI: NM_002745 NP_002736.3