Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GLK, a KHS protein kinase, activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of MEKK1. The protein has a structure similar to other GCK (Group I) kinases and contains an endophilin consensus-binding sequence PPRPPPPR, which has been shown to bind the SH3 domain of endophilin I in rat. GLK is activated by UV radiation and the proinflammatory cytokine tumor necrosis factor (TNF)-alpha.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-GLK antibody in HeLa cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
Requested From: United States
Date Requested: 10/21/2016