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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP4K1 / HPK1 Antibody (clone 2A1) IHC‑plus™ LS‑B6261 Best Seller
Note: This antibody replaces LS-C62065
HPK1 antibody LS-B6261 is an unconjugated mouse monoclonal antibody to human HPK1 (MAP4K1). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6261-50
50 µl (2 mg/ml)
Unavailable

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Product Description

HPK1 antibody LS-B6261 is an unconjugated mouse monoclonal antibody to human HPK1 (MAP4K1). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About MAP4K1 / HPK1
HPK1, a KHS protein kinase, is an upstream activator of the stress-activated protein kinase (SAPK) signaling pathway. The protein has a structure similar to other GCK (Group I) kinases and contains four consensus SH3 binding motifs, which have been shown to interact with the SH3 motifs of the MAP kinase kinase kinases MLK3 and MEKK1 and several adapter proteins that couple to tyrosine kinases, including BASH, Blnk, Clnk, Crk, CrkL and Grb2. Q92918 NM_007181 NP_009112.1

MAP4K1 Antibody, HPK1 Antibody, MEKKK 1 Antibody, MEK kinase kinase 1 Antibody, MHPK1 Antibody


Specifications

Target
Human MAP4K1 / HPK1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,l Monoclonal [2A1]
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:5000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAP4K1 / HPK1 antibody was raised against recombinant human protein purified from E. coli (His/ABD-HPK1).
Presentation
HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Sourced in Ascites.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.
Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.

Western blot

Immunohistochemistry

Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.
Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.

Western blot

Immunohistochemistry

Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.
Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.

Western blot

Immunohistochemistry

Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.
Anti-MAP4K1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6261 dilution 1:200.

Western blot



Requested From: 
Date Requested: 6/21/2018

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