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Anti-MAP3K5 / ASK1 Antibody (phospho-Ser83) LS-C18846


Wt. Vol. Conc. Price
200 µg - - Unavailable

Most Popular MAP3K5 / ASK1 Antibodies

Anti-MAP3K5 / ASK1 Antibody (Ser83) LS-C98720
Rabbit Polyclonal to Human MAP3K5 / ASK1
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-MAP3K5 / ASK1 Antibody (C-Terminus) IHC-plus™ LS-B3205
Rabbit Polyclonal to Human MAP3K5 / ASK1
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Immunohistochemistry Image
Anti-MAP3K5 / ASK1 Antibody (N-Terminus) IHC-plus™ LS-B3206
Rabbit Polyclonal to Human MAP3K5 / ASK1
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human MAP3K5 / ASK1
Western blot, ELISA


Human MAP3K5 / ASK1
Human (tested or 100% immunogen sequence identity)
Purified IgG


  • Western blot (1:1000)
  • ELISA (1:5000 - 1:10000)

Specificity and Use

KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below.
For research use only.

Publications (1)

Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17. [PubMed:20048146] [PMC:PMC2825405]

About MAP3K5 / ASK1

Q99683 NM_005923 NP_005914.1

MAP3K5 Antibody, ASK-1 Antibody, ASK1 Antibody, MEKK5 Antibody, MEK kinase 5 Antibody, MEKK 5 Antibody, MAPK/ERK kinase kinase 5 Antibody, MAPKKK5 Antibody, MAP/ERK kinase kinase 5 Antibody

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation.

Western blot

Western blot
Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

Requested From: 
Date Requested: 3/26/2017

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