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Catalog Number	Size	Price
LS-C18846-200	200 µg (1 mg/ml)	$567

MAP3K5 / ASK1 Antibody - Anti-ASK-1 phospho specific pS83 Antibody - Western Blot. Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody was tested by western blot against Cos-7 cell lysates after transient transfection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 ul of 1x SDS-sample buffer, heated at 96?C for 5, and vortexed for 30 sec. Samples (10 ul each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with anti-HA antibody. This blot demonstrates both recombinant transfections express rASK1. In panel b) all samples were incubated with anti-pS83 ASK1. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with anti-pS83 ASK1 antibody as before except the antibody was pre-incubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.

MAP3K5 / ASK1 Antibody - Anti-ASK-1 phospho specific pS83 Antibody - ELISA. ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Rabbit IgG H&L and TMB substrate were used for detection.

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Polyclonal Rabbit anti‑Human MAP3K5 / ASK1 Antibody (phospho‑Ser83, WB) LS‑C18846

Name: Anti-MAP3K5 / ASK1 Antibody | Rabbit anti-Human Polyclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C18846-200
Price: 567.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

MAP3K5 / ASK1 Rabbit anti-Human Polyclonal (pSer83) Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$567

LS-C18846-200

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAP3K5 / ASK1 Rabbit anti-Human Polyclonal (pSer83) Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

ASK1 antibody LS-C18846 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5) (pSer83). Validated for ELISA and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human MAP3K5 / ASK1

Synonyms

See All MAP3K5 / ASK1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Modifications

Unmodified

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to an internal region near amino acids 75-100 of human ASK-1 protein.

Epitope

pSer83

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.

Applications

Western blot (1:1000)
ELISA (1:5000 - 1:10000)

Usage

This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP3K5 / ASK1

Uniprot: Q99683 NCBI: NM_005923 NP_005914.1

LSBio Ratings

MAP3K5 / ASK1 Antibody (phospho-Ser83) for WB/Western, ELISA LS-C18846 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17.

PubMed: 20048146 PMC: PMC2825405

Submit a Publication for LS-C18846