MKK4 antibody LS-C16098 is an unconjugated rabbit polyclonal antibody to MKK4 (MAP2K4) from human, mouse and rat. Validated for WB.
Human MAP2K4 / MKK4
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
MAP2K4 / MKK4 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr261 of human SEK1.
Detects endogenous levels of human SEK1/ MKK4 protein only when phosphorylated at threonine 261. Does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. Species cross-reactivity: Mouse and rat.
Western Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.