Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 5H4] (IgG1) to Human MAP2K4 / MKK4
IHC, IHC - Paraffin, Flow Cytometry, ELISA
Rabbit Polyclonal (IgG) to Human MAP2K4 / MKK4
Human, Mouse, Rat
Human MAP2K4 / MKK4
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:2000)
Specificity and Use
MAP2K4 / MKK4 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr261 of human SEK1.
Detects endogenous levels of human SEK1/ MKK4 protein only when phosphorylated at threonine 261. Does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. Species cross-reactivity: Mouse and rat.
Western Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.