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Catalog Number Size Price
LS-C368409-30 30 µl (1 mg/ml) $263 
LS-C368409-100 100 µl (1 mg/ml) $315 
LS-C368409-200 200 µl (1 mg/ml) $379 
MAP2K3 / MEK3 / MKK3 Antibody - Immunohistochemical analysis of MKK3 (pT222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MAP2K3 / MEK3 / MKK3 Antibody - Immunofluorescent analysis of MKK3 (pT222) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MAP2K3 / MEK3 / MKK3 Antibody - Western blot analysis of MKK3 (pT222) expression in HeLa (A); Jurkat (B) whole cell lysates.
MAP2K3 / MEK3 / MKK3 Antibody - Immunohistochemical analysis of MKK3 (pT222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MAP2K3 / MEK3 / MKK3 Antibody - Immunofluorescent analysis of MKK3 (pT222) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MAP2K3 / MEK3 / MKK3 Antibody - Western blot analysis of MKK3 (pT222) expression in HeLa (A); Jurkat (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human MAP2K3 / MEK3 / MKK3 Antibody (phospho‑Thr222, IHC, IF, WB) LS‑C368409

Polyclonal Rabbit anti‑Human MAP2K3 / MEK3 / MKK3 Antibody (phospho‑Thr222, IHC, IF, WB) LS‑C368409

Antibody:
MAP2K3 / MEK3 / MKK3 Rabbit anti-Human Polyclonal (pThr222) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C368409-30
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAP2K3 / MEK3 / MKK3 Rabbit anti-Human Polyclonal (pThr222) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
MKK3 antibody LS-C368409 is an unconjugated rabbit polyclonal antibody to MKK3 (MAP2K3 / MEK3) (pThr222) from human. It is reactive with human, mouse and zebrafish. Validated for ICC, IF, IHC and WB.
Target
Human MAP2K3 / MEK3 / MKK3
Synonyms
MAP2K3 | MAPKK 3 | MAPKK3 | PRKMK3 | SAPK kinase 2 | SAPKK-2 | SAPKK2 | SKK2 | MAP kinase kinase 3 | MEK 3 | MEK3 | MKK3 | MAPK/ERK kinase 3
Host
Rabbit
Reactivity
Human, Mouse, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human MKK3.
Epitope
pThr222
Specificity
Recognizes endogenous levels of MKK3 (pT222) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K3 / MEK3 / MKK3
P46734 NM_002756 NP_002747.2

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Requested From: United States
Date Requested: 4/25/2024