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Anti-MAP2K3 / MEK3 / MKK3 Antibody (phospho-Thr222) LS-C199213

Catalog Size Price
LS-C199213-50 50 µl Unavailable
LS-C199213-100 100 µl Unavailable

Most Popular MAP2K3 / MEK3 / MKK3 Antibodies

Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 1D10) IHC-plus™ LS-B5521
Mouse Monoclonal [clone 1D10] (IgG1,k) to Human MAP2K3 / MEK3 / MKK3
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-MAP2K3 / MEK3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5521 concentration 5 ug/ml.
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 2A7) LS-C337935
Mouse Monoclonal [clone 2A7] (IgG2b) to Human MAP2K3 / MEK3 / MKK3
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 6B11) LS-C337938
Mouse Monoclonal [clone 6B11] (IgG2b) to Human MAP2K3 / MEK3 / MKK3
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-MAP2K3 / MEK3 / MKK3 Antibody (phospho-Thr222) LS-C368409
Rabbit Polyclonal to Human MAP2K3 / MEK3 / MKK3
Human, Mouse, Zebrafish
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of MKK3 (pT222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 2E12D11) LS-C669433
Mouse Monoclonal [clone 2E12D11] (IgG1) to Human MAP2K3 / MEK3 / MKK3
Human
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human MAP2K3 / MEK3 / MKK3
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MAP2K3 / MEK3 / MKK3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:1000)

Specificity and Use

Synthetic peptide derived from human MAP2K3 around the phosphorylation site of Thr222.
pThr222
MAP2K3 (Phospho-Thr222) Antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine222.

Packaging

PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C.
For research use only.

About MAP2K3 / MEK3 / MKK3

P46734 NM_002756 NP_002747.2

MAP2K3 Antibody, MAPKK 3 Antibody, MAPKK3 Antibody, PRKMK3 Antibody, SAPK kinase 2 Antibody, SAPKK-2 Antibody, SAPKK2 Antibody, SKK2 Antibody, MAP kinase kinase 3 Antibody, MEK 3 Antibody, MEK3 Antibody, MKK3 Antibody, MAPK/ERK kinase 3 Antibody

MAP2K3 / MEK3 / MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter.

Immunohistochemistry

IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.

Requested From: 
Date Requested: 4/24/2018

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