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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP2K3 / MEK3 / MKK3 Antibody (phospho‑Thr222) LS‑C199213
MKK3 antibody LS-C199213 is an unconjugated rabbit polyclonal antibody to MKK3 (MAP2K3 / MEK3) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199213-50
50 µl
Unavailable
LS-C199213-100
100 µl
Unavailable

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Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

MKK3 antibody LS-C199213 is an unconjugated rabbit polyclonal antibody to MKK3 (MAP2K3 / MEK3) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About MAP2K3 / MEK3 / MKK3
MAP2K3 / MEK3 / MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. P46734 NM_002756 NP_002747.2

MAP2K3 Antibody, MAPKK 3 Antibody, MAPKK3 Antibody, PRKMK3 Antibody, SAPK kinase 2 Antibody, SAPKK-2 Antibody, SAPKK2 Antibody, SKK2 Antibody, MAP kinase kinase 3 Antibody, MEK 3 Antibody, MEK3 Antibody, MKK3 Antibody, MAPK/ERK kinase 3 Antibody


Specifications

Target
Human MAP2K3 / MEK3 / MKK3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from human MAP2K3 around the phosphorylation site of Thr222.
Epitope
pThr222
Specificity
MAP2K3 (Phospho-Thr222) Antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine222.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/22/2018

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